sirtuin 6  (Proteintech)

Journal: Cell Death & Disease

Article Title: Mechanisms of chemotherapy failure in refractory/relapsed acute myeloid leukemia: the role of cytarabine resistance and mitochondrial metabolism

doi: 10.1038/s41419-025-07653-6

Figure Lengend Snippet: A Venn diagram showing relationships between upregulated proteins and upregulated phosphoproteins. B Bar-charts showing the overlap between significantly enriched gene ontology biological processes (GOBPs) by differentially expressed proteins (DEPs) and phosphor-DEPs. C Network model describing the cellular pathways of upregulated/downregulated proteins or phosphorylation in RHI-1 cells. The diagram shows the regulation and phosphorylation status of protein under three conditions: RHI-1(untreated), RHI-1(0.61 Comb), and RHI-1(2 Comb). The large triangles represent protein expression levels, and the small triangles represent phosphorylation levels. (Red-colored large triangles indicate up-regulation, and red-colored small triangles indicate up-phosphorylation. Blue-colored one is down-regulation. Ara-C and DNR are denoted by “A” and “D”, respectively. D – F Quantification of the levels of SIRT6 ( D ), phosphor-DNAPKcs ( E ) and CAT ( F ) using immunoblotting. Bar charts represent mean ± standard deviation (S.D.) of values derived from three independent experiments.

Article Snippet: The following antibodies were used at a dilution of 1:1000, rabbit anti-Sirtuin 6 (SIRT6) antibody (cell signaling technology, Beverly, MA, USA; RRID:AB_2188926), rabbit anti-catalase (CAT) antibody (cell signaling technology, Beverly, MA, USA; RRID:AB_2798079), rabbit anti-phosphoDNA-dependent protein kinase (phos-DNA-PK) antibody (cell signaling technology, Beverly, MA, USA; RRID:AB_2939025), and β-actin (Invitrogen).

Techniques: Phospho-proteomics, Expressing, Western Blot, Standard Deviation, Derivative Assay

Journal: Cell Death & Disease

Article Title: Mechanisms of chemotherapy failure in refractory/relapsed acute myeloid leukemia: the role of cytarabine resistance and mitochondrial metabolism

doi: 10.1038/s41419-025-07653-6

Figure Lengend Snippet: A Box plots of cell viability of under (i) 0.64 μM Ara-C, (ii) 0.61 μM, and (iii) 2 μM DNR with SIRT6 inhibitor. B Western blot analysis confirming siRNA-mediated knockdown of SIRT6 in RHI-1 cells. Cellular ATP levels in SHI-1 and RHI-1 cells under drug treatment. C Box plots of cell viability under (i) 0.64 μM Ara-C, (ii) 0.61 μM, and (iii)) 2 μM DNR with GPD2 inhibitor and D rotenone. E Box plots of cell viability of patient samples under (i) 0.64 μM Ara-C, (ii) 0.61 μM, and (iii) 2 μM DNR with SIRT6 inhibitor and F rotenone. Box plots represent mean ± standard deviation (S.D.) of values derived from over six independent experiments.

Article Snippet: The following antibodies were used at a dilution of 1:1000, rabbit anti-Sirtuin 6 (SIRT6) antibody (cell signaling technology, Beverly, MA, USA; RRID:AB_2188926), rabbit anti-catalase (CAT) antibody (cell signaling technology, Beverly, MA, USA; RRID:AB_2798079), rabbit anti-phosphoDNA-dependent protein kinase (phos-DNA-PK) antibody (cell signaling technology, Beverly, MA, USA; RRID:AB_2939025), and β-actin (Invitrogen).

Techniques: Western Blot, Knockdown, Standard Deviation, Derivative Assay